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. 2012 Jan 10;31(5):1190–1202. doi: 10.1038/emboj.2011.486

Figure 6.

Figure 6

Id1–Id4 knockdown rescued the precocious astrogenesis defects and promoted neuronal differentiation in Rp58 KO mice. (A–F″) Representative images of coronal sections of Rp58 KO cortices electroporated at E14.5 with control scramble (Sc) shRNA (A, C–C″, E–E″) or co-electroporated with Sc shRNA and shId1, shId2, shId3, or shId4 (B, D–D″, F–F″) and fixed at E18.5 and stained with anti-GFP (green, A, B, C, D, C″, D″, E, F, E″, F″) and anti-GFAP (red, C′, D′, C″, D″), anti-Sox2 (blue, E′, F′), and anti-Tbr1 (red, E, F, E″, F″). Scale bars: 25 μm. (G, H) Quantification of the ratio of GFAP-positive cells to GFP-positive cells in (C″, D″, G), and the ratio of Tbr1-positive cells to GFP-positive cells in (E″, F″, H) (t-test: *P<0.05, **P<0.01). Error bars indicate s.d. (n=3). The arrows in (C″) indicate the double-stained cells.