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. 2012 Jan 13;31(5):1080–1094. doi: 10.1038/emboj.2011.492

Figure 4.

Figure 4

Reduction of the lamin B1 levels rescues nuclear shape alteration and senescence in A-T primary fibroblasts. (A) Western blot analysis of lamin B1 in extract from A-T primary fibroblasts transfected with control or lamin B1 siRNA (20 nM). (B) The alterations in nuclear shape in A-T human primary fibroblasts transfected with control or lamin B1 siRNA were examined by immunofluorescence with anti-lamin B1 (red) and DAPI (blue) 72 h following transfection (left panel). Right panel: % of misshapen nuclei (circularity ⩽0.65) from three independent experiments. Nuclear shape analysis was performed on at least 100 cells per condition. (C) The SA-β-gal assay was performed on A-T human primary fibroblasts (GM05823) 72 h after transfection with control or lamin B1 siRNA. Histograms represent the quantification of SA-β-gal-positive cells from more than five randomly chosen fields (× 10 magnification). *Represents a statistically significant difference (P<0.05). Error bars denote the s.e.m.