Figure 5.

Effect of oxidative stress on lamin B1 levels and nuclear morphology. (A) WT and A-T lymphoblasts were treated with H₂O₂ or BSO and NAC, respectively. (B) WT and A-T primary fibroblasts were treated with H₂O₂ and NAC, respectively. Upper right panels: quantification of lamin B1 levels relative to actin following treatment. The values correspond to the means of three to eight independent experiments. *Represents a statistically significant difference (P<0.05) between untreated (NT) and treated cells. The error bars denote the s.e.m. (C) Impact of oxidative stress on nuclear shape. Upper panels: WT and A-T fibroblasts were treated with H₂O₂ and NAC, respectively, for 72 h and analysed by immunofluorescence with anti-lamin B1 (green) and DAPI (blue). White arrows: alterations in nuclear morphology (white arrows). Lower panels: quantification of abnormal nuclei. Means from three separate experiments are expressed after H₂O₂ treatment in wild-type primary fibroblasts (GM03349 p14 and GM03652 p14, left panel) and after NAC treatment in A-T primary fibroblasts (GM2052 p14 and GM05823 p18, right panel). At least 150 cells were counted per group. *Represents a statistically significant difference (P<0.05) between untreated (NT) versus treated cells. The error bars denote the s.e.m.