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. 2012 Mar 7;9(3):216–224. doi: 10.7150/ijms.4004

Figure 1.

Figure 1

Rapamycin inhibits the phosphorylation of mTOR and siSTAT3 specifically knocks down the level of STAT3 in Bel-7402 cells. A. Cells were treated without (Ctrl) or with 109 nmol/L rapamycin (Rap) for 48 h. The expression levels of phosphorylated mTOR (p-mTOR), total mTOR, and β-actin (as internal control) were examined by Western blotting. A representative image is shown on the left, and quantifications of p-mTOR/β-actin as well as mTOR/β-actin on the right. *P < 0.05, as compared to Ctrl cells. B. Cells were transfected with either pGC-siSTAT3 or pGC-siCtrl. The expression level of STAT3 was determined by Western blotting and compared to that in non-treated cells (Ctrl). A representative image is shown on the left, and quantification of STAT3/β-actin as well as mTOR/β-actin on the right. *P < 0.05, as compared to Ctrl cells.