Table 1. Protein changes in DOX-treated adult rat cardiomyocytes.
Proteins identified to undergo changes due to 48 h DOX treatment. Some of the unknown proteins are included since their mass spectrum is very good yet the data base search does not yield a significant ID.
| Accession # | Molecular function | Matched / Measured peptides | score | ||
|---|---|---|---|---|---|
| Upregulated proteins (DOX treated cardiomyocytes) | |||||
| Spot # 1 | Alpha B-crystallin (19.9KDa, pl 6.8) | CAA42911 | Structural protein / Molecular chaperone | 11 / 46 - 22% coverage | 68 |
| Spot # 2 | Alpha B-crystallin (19.9KDa, pl 6.8) | AAP31996 | Structural protein / Molecular chaperone | 11 / 46 - 22% coverage | 68 |
| Spot # 3 | Potassium dependent sodium-calcium exchanger (NCKX2) (19.7KDa, pl 6.5) | NP_113931 | Transporter | 26/44 - 54% coverage | 212 |
| Spot # 4 | DNA damage inducible transcript 4-like / SMHS1 (Ddit4) (21.9KDa, pl 6.8) | NP_536324 | Energy metabolism / apoptosis regulator | 9 / 33 - 12% coverage | 64 |
| Spot # 9 | Troponin-T (TnnT2) (35.7KDa, pl 5) | NP_036808 | Actin binding motor protein | 16 / 46 -36% coverage | 116 |
| Spot # 11 | Protease (prosome, macropain) 28 subunit, alpha (PSME1) (28.8KDa, pl 5.6) | AAH60574 | Proteosome activator | 6 / 42 - 17% coverage | 74 |
| Spot # 19 | Translocation protein 1 / AB2-292 (Tloc1, 45.9KDa, pl 6.9) | NP_001029301 | Protein transporter activity | 8/28 - 16% coverage | 71 |
| Spot # 22 | MTSG1 346 amino acid isoform (Mtusl, 40.2KDa, pl 5.8) | AAO60218 | Mitochondrial tumor suppressor gene may be involved in regulating cell proliferation | 17 / 42 - 34% coverage | 124 |
| Spot # 23 | MTSG1 346 amino acid isoform (Mtusl, 40.2KDa, pl 5.8) | AAO60218 | Mitochondrial tumor suppressor gene may be involved in regulating cell proliferation | 17 / 43 - 34% coverage | 124 |
| Spot # 24 | Lamin C2 (Lmna, 52.6KDa, pl 6.2) | CAA67641 | Intermediate filament (structural protein) | 9 / 29 - 20% coverage | 194 |
| Spot # 25 | Electron transfer flavoprotein ubiquinone oxidoreductase precursor (ETFDH, 68.1KDa, pl 7.4) | AAQ67364 | Oxidoreductase | 8 / 42 - 16% coverage | 71 |
| Spot # 26 | Stress induced phosphoprotein I (Stipl, 63.2 Kda, pl 6.4) | AAH61529 | Chaperone | 14 / 63 - 22% coverage | 92 |
| Spot # 29 | MAP kinase 14 / p38 alpha (MAPK14, 40KDa, pl 5.9) | NP_112282 | Non receptor serine threonine protein kinase | 12 / 36 - 28% coverage | 107 |
| Spot # 31 | F1-ATPase beta subunit (ATP5b, 38.8KDa, pl 5.1) | AAA40778 | Ion channel hydrogen transporter | 15 / 29 - 45% coverage | 170 |
| Spot # 34 | Lactate dehydrogenase B (Ldhb, 36.9KDa, pl 5.7) | AAH59149 | Dehydrogenase (oxidoreductase) | 10 / 44 - 26% coverage | 64 |
| Differentially upregulated proteins (DOX treated cardiomyocytes) | |||||
| DUR # 11 | Beta enolase (ENO3, 47KDa, pl 7.53) | CAA68788 | Lyase | 19 / 48 - 40% coverage | 173 |
| DUR # 14 | F1- ATPase beta subunit (ATP5b, 56.3KDa, pl 4.9) | AAA40778 | ATP synthase | 9 / 48 - 24% coverage | 104 |
| DUR # 15 | Actin gamma -2 (Actg2, pl 5.2) | NP_037025 | Actin and actin related protein | 7 / 38 - 17% coverage | 97 |
| DUR # 16 | Enolase alpha (Eno1, pl 6.5) | AAH78896 | Lyase | 18 / 38 - 43% coverage | 176 |
| DUR # 17 | Lipocortin V (Anxa5, pl 4.65) | AAC06290 | Transfer/carrier protein | 10 / 32 - 26% coverage | 158 |
| DUR # 18 | Creatine kinase (Ckm, 43KDa, pl 7.1) | AAA40936 | Transferase | 7 / 46 - 14% coverage | 148 |
| DUR # 20 | Lactate dehydrogenase B (Ldhb, pl 5.92) | AAH59149 | Dehydrogenase | 8 / 44 - 22% coverage | 159 |
| Differentially downregulated proteins (DOX treated cardiomyocytes) | |||||
| DDR # 21 | ATP synthase, H+ transporting, mitochondrial F1 complex, beta precursor (Atp5b, 56.3KDa, pl 4.92) | P10719 | ATP synthase | 21 / 45 - 60% coverage | 209 |
| DDR # 22 | Electron transfer flavoprotein beta subunit (ETF-β, pl 7.9) | NP_001004220 | Hydroxylase | 11 / 29 - 44% coverage | 110 |
| DDR # 25 | Methylmalonate semialdehyde dehydrogenase (MMSDH, ALDH6A1, 57.8KDa, pl 7.9) | Q02253 | Aldehyde dehydrogenase member | 16 / 30 - 34% coverage | 163 |
| DDR # 26 | Voltage dependent anion selective channel protein 1 (Vdac1, pl 8.6) | AAH72484 | Anion channel / Voltage gated ion channel | 10 / 29 - 54% coverage | 201 |
| Downregulated proteins (DOX treated cardiomyocytes) | |||||
| DR # 27 | Voltage dependent anion selective channel protein 2 (Vdac2, 31.8KDapl 7.5) | P81155 | Anion channel / Voltage gated ion channel | 8 / 77 - 34% coverage | 74 |
| DR # 28 | Pleckstrin homology domain containing family A (PI binding specific) member 8 (Plekha8, pl 4.7) | NP_001102705 | Nucleic acid binding / Transfer protein | 6 / 41 - 28% coverage | 81 |
Protein score is -10*log(P) where P is the probability that the observed match is a random event.
Protein scores greater than 61 are significant (P<0.05).