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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Int J Pharm. 2011 Dec 6;423(2):525–534. doi: 10.1016/j.ijpharm.2011.11.040

Fig. 4.

Fig. 4

Colony selection of HCV (+) replicon cells transfected with different siRNA nanosomes and with IFN at specific time points. After 24 hours culture in 6-well plates, HCV (+) replicon cells were transfected with different formulations or with IFN for 48 hours. The cells were scraped and re-cultured for 72 hours and then were transfected for a second time with the same formulations, or with IFN for 48 hours. Finally the cells were scraped and re-plated in a 100 mm dish and cultured in the presence of G-418 to select HCV (+) cell colonies that were not inhibited by siRNA nanosomes.