Fig. 5.

Cell viability of R4GFP HCV (+) replicon cells transfected with different siRNA nanosomes and IFN at specific time points. After 24 hours culture in 24-well TCPs, the cells were transfected with different formulations, or with IFN, for another 48 hours. Subsequently, the cells were incubated with MTT solution at 37°C for 3 hours, and the absorbance reading was taken at 570 nm. The percentage of cell viability was determined by comparing viable cells to the untreated cells during transfection at different time points. The results represent mean ± standard deviation (n=5).