Table 1.
Deduced functions of ORFs in the herboxidiene biosynthetic gene cluster
| Gene | No. of amino acids | Protein homologue (accession no.) | % Identity/similarity | Proposed function(s) |
|---|---|---|---|---|
| orf1 | 269 | HypB of Rhodococcus jostii RHA1 (ABG96388) | 74/88 | Hydrogenase nickel incorporation protein HypB |
| orf2 | 340 | MviM of Streptomyces bingchenggensis BCW-1 (ADI12327) | 82/90 | Oxidoreductase domain protein |
| orf3 | 261 | Hfi of Streptomyces violaceusniger Tü 4113 (AEM84847) | 86/90 | Xylose isomerase domain protein |
| herA | 341 | PurR of S. violaceusniger Tü 4113 (YP_004815128) | 77/83 | Transcriptional regulator, LacI family |
| herB | 6394 | PldAI of Streptomyces platensis Mer-11107 (BAH02268) | 56/67 | Type I polyketide synthase |
| Loading module | KS, AT, ACP | |||
| Module 1 | KS, AT, KR, ACP | |||
| Module 2 | KS, AT, DH, KR, ACP | |||
| Module 3 | KS, AT, DH, ER, KR, ACP | |||
| herC | 7138 | PldAII of S. platensis Mer-11107 (BAH02269) | 56/66 | Type I polyketide synthase |
| Module 4 | KS, AT, DH, KR, ACP | |||
| Module 5 | KS, AT, DH, KR, ACP | |||
| Module 6 | KS, AT, KR, ACP | |||
| Module 7 | KS, AT, DH, ER, KR, ACP | |||
| herD | 2030 | ORF17 of Streptomyces aizunensis NRRL B-11277 (AAX98192) | 55/65 | Type I polyketide synthase |
| Module 8 | KS, AT, DH, KR, ACP, TE | |||
| herE | 477 | PldD of S. platensis Mer-11107 (BAH02274) | 57/70 | Epoxidase |
| herF | 297 | MitM of Streptomyces lavendulae NRRL 2564 (AF127374_14) | 48/61 | O-Methyltransferase |
| herG | 422 | P450 of S. platensis (CBX53644) | 50/63 | Cytochrome P450 monooxygenase |
| orf4 | 177 | NAT_SF of Streptomyces griseoaurantiacus M045(ZP_08289959) | 66/74 | GCN5-like N-acetyltransferase |
| orf5 | 422 | SSFG-07305 of Streptomyces ghanaensis ATCC 14672 (ZP_06581609) | 65/78 | Hypothetical protein |
| orf6 | 278 | SSFG_07304 of S. ghanaensis ATCC 14672 (ZP_06581608) | 62/76 | Abhydrolase |