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The mutated aacCm cassette is resistant to the cleavage of T. denticola. PCR amplified 534 bp of the wild-type aacC1 cassette, and mutated aacCm fragments were treated with the crude cell extract of Td35405. The samples obtained were then analyzed by 2% agarose gel electrophoresis and visualized with ethidium bromide staining. Lane M contains DNA markers. The two white arrows point toward the cleaved products.
