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. 2012 Mar;78(6):2059–2062. doi: 10.1128/AEM.07461-11

Fig 2.

Fig 2

Schematic illustration of constructing the PrcA::aacCm vector for the deletion of prcA gene. The vector was constructed via three PCR amplification steps. Arrows indicate the relative positions of PCR primers for constructing the vector. The sequences of these primers are listed in Table 1.