Table 1.
Oligonucleotide primers used in this study
| Primer | Sequencea | Note | Directionb |
|---|---|---|---|
| P1 | CCGAATCTCGGCACCTGT | 5′-Flanking region of prcA | F |
| P2 | GCGTAACATACTCGTCCTCCTATAAAT | 5′-Flanking region of prcA | R |
| P3 | GGAGGACGAGTATGTTACGCAGCAGCAACG | aacCm cassette | F |
| P4 | GACCTCCTTAGGTGGCGGTACTTGGG | aacCm cassette | R |
| P5 | GCCACCTAAGGAGGTCTTTAAGATGAAG | 3′-Flanking region of prcA | F |
| P6 | GATAGTGCCCGAACAGTG | 3′-Flanking region of prcA | R |
| P7 | ATGTTACGCAGCAGCAACG | aacCm | F |
| P8 | TTAGGTGGCGGTACTTGGG | aacCm | R |
| P9 | GCTGCGGCGTTATGGTGT | Detected prcA | F |
| P10 | CCGTCGTCGAGGGGAAAG | Detected prcA | R |
| P11 | GCAGCGGGTACAAAGATAC | 5′-Flanking region of prcB | F |
a
The underlined sequences are engineered overlapping sequences for PCR amplifications.
b
F, forward; R, reverse.