Table 5.
Summary of qPCR analysis of M. gallisepticum dilution series
| Dilution | Avg crossing threshold valuea |
|---|---|
| 100 | 11.18 ± 0.14 |
| 10−1 | 14.72 ± 0.16 |
| 10−2 | 17.44 ± 0.25 |
| 10−3 | 20.88 ± 0.08 |
| 10−4 | 24.85 ± 0.04 |
| 10−5 | 27.00 ± 0.10 |
| 10−6 | 30.47 ± 0.38 |
| 10−7 | 31.56 ± 0.32 |
| 10−8 | 32.33 ± 0.17 |
| 10−9 | 31.96 ± 0.12 |
| 10−10 | 31.67 ± 0.01 |
a
Regression analysis of the starting concentrations versus the threshold cycle gave an R2 value of 99% for the first eight dilutions (100 to 10−8). The 10−9 and 10−10 dilutions were recognized by the qPCR (mglp) primers but did not exhibit a linear relationship between threshold number and concentration.