Table 1.
Primers used for PCR amplification of cry19A and orf2 sequences
| Primer | Sequencea |
|---|---|
| 19A-R | 5′-CGGGATCCATTCACAACCTTTTTTCTTATTTT-3′ |
| OPN-19F | 5′-AAGAAACATTATTATGGGAATAGG-3′ |
| OPN-19R | 5′-CGTTCCTCCCTTATTCCCATCATT-3′ |
| OPN-F | 5′-AACTGCAGGCATGCAACAGAACCCTAAAAAAT-3′ |
| OPN-ORF2F | 5′-TTTAAACTTACAAGTGGTGCGAAA-3′ |
| OPN-ORF2R | 5′-ATTCACAACCTTTTTTCTTATTTT-3′ |
| OPN-R | 5′-CGGGATCCATGCATAGATTCGTAATAGTATCT-3′ |
| ORF2-F | 5′-TTTATGCTTACAAGTGGTGCGAAA-3′ |
a
Restriction endonuclease cleavage sites for BamHI and PstI are underlined; intact and mutated ATG codons are shown in boldface and shaded, respectively.