Table 2.
Primers used for amplification of different constructs of cry19A, orf2, and cry1C
| Primer | Sequencea |
|---|---|
| 1C-1 | 5′-GCGGTGAATGCCCTGTTTAC-3′ |
| 1C-2 | 5′-ACATGCATGCCCCCTTAGATAGATATCATAG-3′ |
| 1C-3 | 5′-TCACTTTTGTGCTC TTTCTAAATCAGA-3′ |
| 1C-4 | 5′-CTTTTGTGCTC TTTCTAAATCAGA-3′ |
| 19A-1 | 5′-ACGCGTCGACGAATAAGGGAGGAACGAAGA-3′ |
| 19A-2 | 5′-AACTGCAGAATGTTTCCTGTGATCTTTC-3′ |
| 19A-3 | 5′-AACTGCAGATTCTATTCTTTTTCAAACT-3′ |
| 19A-4 | 5′-GTTAGTTGGGAGGAATTCGA-3′ |
| DS-1 | 5′-GGAATTCTATTTTCGATTTC-3′ |
| ORF2-1 | 5′-TACCATTCACAGGAAATATG-3′ |
| ORF2-2 | 5′-ATGCTTACAAGTGGTGCGAAAAATATGTTA-3′ |
a
The ribosome binding site and artificial stop codon (bold type) and the SphI, SalI, PstI, and EcoRI sites used for cloning (underlined) are shown.