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PCR analyses of the single and double recombinants. The numbers on the left indicate the sizes of some fragments based on the molecular size marker (lanes M). The primer pairs and templates for each reaction are indicated above the corresponding lane. Lanes WT and MT, wild type and pfkB mutant of A. ferrooxidans ATCC 23270, respectively; lanes Af1 and Af2, the two types of single recombinants in Fig. 1, respectively.
