Table 1.

PCR primers used for determination of L. monocytogenes virulence marker genes

PCR test	Primer name	Sequence (5′→3′)	Primer final concn (μM)	Target gene	Size of PCR amplicon (bp)	Reference
mPCR1	inlAF	ACGAGTAACGGGACAAATGC	0.25	inlA	800	11
	inlAR	CCCGACAGTGGTGCTAGATT	0.25
	inlCF	AATTCCCACAGGACACAACC	0.15	inlC	517
	inlCR	CGGGAATGCAATTTTTCACTA	0.15
	inlJF	TGTAACCCCGCTTACACACAGTT	0.05	inlJ	238
	inlJR	AGCGGCTTGGCAGTCTAATA	0.05
PCR2	lmo2672F	CGGCACACTTGGATTCTCAT	0.3	lmo2672	481	10
	lmo2672R	AGGGCTAGTGACGGATGCTA	0.3
PCR3	llsXF	TTATTGCATCAATTGTTCTAGGG	0.2	llsX	200	1
	llsXR	CCCCTATAAACATCATGCTAGTG	0.2