Table 1.
P. freudenreichii WT strains and their genetically modified derivatives used in this study
| Strain or plasmid | Relevant genotype and phenotype | Source or reference |
|---|---|---|
| Strains | ||
| CB1a (formerly TL34) | WT, carrying one genomic copy of gtfF and producing β-glucan | 17 |
| CB1-KO | Inactivation of gtfF gene | 14 |
| CB1-KO-C | CB1-KO complemented with gtfF and with reverted phenotype | This study |
| LSP110 (formerly TL162) | WT, carrying one genomic copy of gtfF and producing β-glucan | 12 |
| LSP110-KO | Inactivation of gtfF gene | This study |
| LSP110-KO-C | CB1-KO reverted to WT | This study |
| LSP103 (formerly TL176) | WT, carrying one genomic copy of gtfF and producing β-glucan | 12 |
| LSP103-KO | Inactivation of gtfF gene | This study |
| LSP103-KO-C | CB1-KO reverted to WT | This study |
| Plasmids | ||
| pUC:gtfF:CmR | pUC18 carrying a chloramphenicol resistance gene and a truncated fragment of gtfF | 14 |
| pPK705 | E. coli-P. freudenreichii shuttle vector, carrying an ampicillin resistance gene | 34 |
| pFB01:gtfF | pPK705 carrying gtfF gene under the control of the strong promoter Ptuf | This study |
a
CB, CIRM-BIA, Centre International de Ressources Microbiennes–Bactéries d'Intérêt Alimentaire, INRA, UMR 1253, Science et Technologie du Lait et de l'Œuf, Rennes, France. The TL collection does not exist anymore and is now included in the CB collection.