Table 2.
Hydrolytic activities of AiiAAI96 against various AHL substratesa
| Substrate | Biosensor bacterium | Enzyme activity (U/ml) |
|---|---|---|
| C4-HSL | KYC55/CV026 | D/44.97 |
| C6-HSL | KYC55/CV026 | D/2.28 |
| C7-HSL | KYC55/CV026 | 1.90/0.39 |
| C8-HSL | KYC55/CV026 | 1.29/0.33 |
| C10-HSL | KYC55 | 11.19 |
| C12-HSL | KYC55 | L |
| C14-HSL | KYC55 | D |
| 3-Oxo-C6-HSL | KYC55/CV026 | L/0.36 |
| 3-Oxo-C8-HSL | KYC55/CV026 | 9.13/8.54 |
| 3-Oxo-C10-HSL | KYC55 | 9.70 |
| 3-Oxo-C12-HSL | KYC55 | 3.08 |
| 3-Oxo-C14-HSL | KYC55 | 312.40 |
| 3-Hydroxy-C8-HSL | KYC55 | 5.22 |
| 3-Hydroxy-C14-HSL | KYC55 | L |
a
KYC55, A. tumefaciens KYC55(pJZ372)(pJZ384)(pJZ410); CV026, C. violaceum CV026; D, degraded but could not be quantified; L, little activity detected.