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Alternative MO injection strategies do not increase the survival beyond P0 ICV MO injection. Three cohorts of mice each received an injection of MO by P0 FV (50 µg/g body mass, n= 3), P0 FV and ICV (50 µg/g peripheral, 54 µg ICV, n= 5) or P0 and P30 ICV (with stereotactic guidance, 54 µg/g P0, 18 µg/g P30, n= 4). Survival for all groups was equivalent to high-dose P0 ICV MO injection (log-rank P> 0.09).
