Table 1. Primers for mutation screening of SAG.
| Exon | F/R | Primer Sequence (5′-3′) | PCR size (bp) |
|---|---|---|---|
| 1 |
F |
GCTTGCATAACACCAGGTTCATC |
750 |
| |
R |
CCGCTCACTCCAAGTCTCC |
|
| 2 |
F |
TTGTCTTACCTTTCTCCAACCC |
255 |
| |
R |
CCCTCAAAGAGTTTTGATGTTG |
|
| 3 |
F |
CATGGATGCCTTAGCTTAGC |
217 |
| |
R |
TAGATTATTAGCAAGGCCAG |
|
| 4 |
F |
GGTTTCTTTCATCTTCTCCA |
189 |
| |
R |
CTCTCCTTCCATGTAAATG |
|
| 5 |
F |
TTGAAAACCCGTGTTCGCTG |
366 |
| |
R |
TCTATCCCCTTTCCTTTGCC |
|
| 6 |
F |
ATATTACTTAATGGAACAGC |
213 |
| |
R |
ACAGAGTAAAACCCTGTTTC |
|
| 7 |
F |
CATGTGCCCTGTGTGAGGTG |
246 |
| |
R |
CCACAGAGACAAGGTGGAGG |
|
| 8 |
F |
GGAGAGAACAGAAGCCTCCC |
300 |
| |
R |
ATGTAGTTAAGGGCTGGGGC |
|
| 9 |
F |
ATTCCAGTGAAAGGGATTGAG |
269 |
| |
R |
ATCAGACCAGAGAAGTGACC |
|
| 10 |
F |
AGGAGAGACCAGCGTGTACC |
240 |
| |
R |
CAGCAATAAACGGCGAGAAAC |
|
| 11 |
F |
GGTCCATGGCAGCTTTGATG |
217 |
| |
R |
CTTATTCCCTGAGCCTCGAG |
|
| 12 |
F |
AAAGGCTGCCCATCTGCTC |
244 |
| |
R |
CCTTGCTTTCTGTCTCCCAG |
|
| 13 |
F |
GAGCTGGGCTGTGTCCTGCC |
173 |
| |
R |
AAGTTTGCTGCCTTTGATAT |
|
| 14 |
F |
GCAGCCATAGGTCTTTGCTG |
258 |
| |
R |
ATGGAATCTCTTACACCTGG |
|
| 15 |
F |
TCAAATTGTAAAGTCACCTAAAAGG |
205 |
| |
R |
AAGAGGGTTTTGTGCTGGAG |
|
| 16 |
F |
CTTGATCAGTTCCTTCGTTGC |
276 |
| R | GACTAAACTGTGGGGCTTTGC |
F, Forward; R, Reverse.