Table 1. Primers Used for Real Time RT–PCR.
| Target Genes | Sequence | Product Size (bp) |
|---|---|---|
|
NCAM1 |
F: 5′-GACTGTCACTCATTCTCCGATCAG-3′ |
82 |
|
|
R: 5′-GTATGATTATTTTTGCAGAATTGTTTCC-3′ |
|
|
TFAP2B |
F: 5′-TTGGGTACATTTGCGAAACG-3′ |
68 |
|
|
R: 5′-TGTGTGCTGCCGGTTCAA-3′ |
|
|
CDK15 |
F: 5′-CACCCAGCCCAGTTTAGCAA-3′ |
73 |
|
|
R: 5′-AAATACAGCCCTGGAAGAACCTT-3′ |
|
|
MEIS1 |
F: 5′-GACAACCTCGCCTGTGATTGA-3′ |
70 |
|
|
R: 5′-CCCCTCAGACCCAACTACCA-3′ |
|
|
COL8A1 |
F: 5′-GGAGATGCCCCACTTGCA-3′ |
72 |
|
|
R: 5′-GCCGGTTGAATTTCCTTCATAT-3′ |
|
|
THY-1 |
F: 5′-ACCTCTTCCTCTTCCCTGACTTC-3′ |
78 |
|
|
R: 5′-GCCCAGTGTGCAGTCATTAGC-3′ |
|
|
CDH2 |
F: 5′-GAGCAGTGAGCCTGCAGATTTT-3′ |
81 |
|
|
R: 5′-TGCTCAGAAGAGAGTGGAAAGCT-3′ |
|
|
TJP1 |
F: 5′-AAAGGAGAGGTGTTCCGTGTTG-3′ |
98 |
|
|
R: 5′-CGTTCTACCTCCTTATGATTTTTACCA-3 |
|
|
GAPDH |
F: 5′-GTATCGTGGAAGGACTCATGACCA-3′ |
126 |
| R: 5′-TAGAGGCAGGGATGATGTTCTGGA-3′ |
The primers were designed with Primer Express 1.5a software (Applied Biosystems). F: forward primer, R: reverse primer.