Table 3.
Type and incidence of histopathological lesions.
| Type of lesion/post-treatment day | Control | Nano TiO2 | Indoor-Nano TiO2 | Indoor-R | Printex 90 |
|---|---|---|---|---|---|
| Foci (small) of inflammatory cells | |||||
| Day 1 | 0/3a | 1/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 1/5 | 0/5 | 0/5 | 0/6 |
| Polymorphonuclear cell foci | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 1/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 2/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 2/6 |
| Hyperplasia of connective tissue perivascular | |||||
| Day 1 | 0/3 | 1/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 1/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 1/5 | 0/5 | 0/5 | 0/6 |
| Hyperplasia of connective tissue near bile ductules or venules | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/5 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 3/6 |
| Necrosis in centrilobular area | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 3/5 | 0/5 | 0/5 | 0/6 |
| Microfoci of necrosis in centrilobular area | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 1/6 |
| Eosinophilic necrosis in single hepatocytes | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 1/6 |
| Hepatocytes with pycnotic nuclei | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 1/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 1/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 1/6 |
| Parenchymal degeneration | |||||
| Day 1 | 0/3 | 0/4 | 0/6 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 2/5 | 0/5 | 0/5 | 3/6 |
| Binucleate hepatocytes | |||||
| Day 1 | 0/3 | 1/4 | 0/6 | 0/5 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 0/5 | 0/5 | 0/5 | 2/6 |
| Oedema of endothelial cells of portal venules | |||||
| Day 1 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 3 | 0/3 | 0/4 | 0/5 | 0/6 | 0/4 |
| Day 28 | 0/5 | 0/5 | 0/6 | 0/5 | 2/6 |
| Increased number of Kupffer cellsb | |||||
| Day 1 | 0/3 | 2/4 | 0/6 | 0/6 | 1/4 |
| Day 3 | 0/3 | 2/4 | 0/5 | 0/6 | 1/4 |
| Day 28 | 0/5 | 2/5 | 0/5 | 0/5 | 2/6 |
Type and incidence of histopathological lesions in the control mice and mice exposed to 162 μg of NanoTiO2, 486 μg Indoor-NanoTiO2, 486 μg Indoor-R, or 162 μg of Printex 90. a: Incidence of a lesion expressed as a number of livers with the lesion of a total number of examined livers. b: Qualitative evaluation only.