Table 1.
The infectivity and pathogenicity of O/CHN/2010/33-OP
| Group | Infected strain | Animals | dpi | Clinic symptom | Virus in OP fluid | Virus in lymph nodes | Virus in spinal cords | 3ABC NSP Ab |
Type O SP Ab (LPB-ELISA) |
|---|---|---|---|---|---|---|---|---|---|
| Group 1 | O/CHN/ 2010/ 33-OP OP fluid |
cattle No.134 |
8 | + | + | 1:720 | |||
| 14 | + | + | + | + | 1:1024 | ||||
| cattle No.184 |
8 | - | - | + | 1:360 | ||||
| 14 | - | - | + | + | 1:1024 | ||||
| Direct contact infection |
pig No.036 |
8 | - | - | < 1:4 | ||||
| 11 | + | - | 1:90 | ||||||
| 14 | + | + | + | 1:512 | |||||
| pig No.039 |
8 | - | - | < 1:4 | |||||
| 11 | - | - | 1:45 | ||||||
| 14 | - | + | - | 1:180 | |||||
| pig No.040 |
8 | - | - | 1:6 | |||||
| 11 | - | - | 1:45 | ||||||
| 14 | - | + | - | 1:128 | |||||
| Group 2 | O/CHN/2010/ 33-OP cell strain |
cattle No.3 |
8 | - | + | 1:90 | |||
| 11 | - | + | 1: 90 | ||||||
| 14 | - | + | + | + | 1: 180 | ||||
| cattle No.146 |
8 | - | - | 1:32 | |||||
| 11 | - | - | 1:90 | ||||||
| 14 | - | + | + | + | 1:128 | ||||
| Direct contact infection |
pig No.041 |
8 | - | - | 1:4 | ||||
| 11 | - | - | 1:4 | ||||||
| 14 | - | + | - | 1:16 | |||||
| pig No.046 |
8 | - | - | 1:12 | |||||
| 11 | - | - | 1:6 | ||||||
| 14 | - | + | - | 1:6 | |||||
| pig No.049 |
8 | - | - | 1:4 | |||||
| 11 | - | - | 1:6 | ||||||
| 14 | - | + | - | 1:6 | |||||
| Group 3 | O/CHN/2010/ 33-OP cell strain |
pig No.029 |
4 | + | / | / | |||
| 5 | + | / | / | ||||||
| 14 | + | + | + | 1:180 | |||||
| pig No.032 |
4 | - | / | / | |||||
| 5 | - | / | / | ||||||
| 14 | - | + | + | 1:512 | |||||
| pig No.033 |
4 | - | / | / | |||||
| 5 | - | / | / | ||||||
| 14 | - | + | + | 1:128 | |||||
| Group 4 (control) |
O/CHN/Mya98/ 2010 cell strain |
cattle No.11 |
5 | - | / | / | |||
| 8 | - | - | 1:32 | ||||||
| 14 | + | + | + | 1:256 | |||||
| cattle No.12 |
5 | + | - | 1:90 | |||||
| 8 | + | + | / | ||||||
| 14 | + | + | + | 1:180 | |||||
| Group 5 (control) |
O/CHN/Mya98/ 2010 cell strain |
pig No.047 |
5 | - | / | / | |||
| 8 | - | / | / | ||||||
| 14 | - | + | + | 1:180 | |||||
| pig No.048 |
5 | - | / | / | |||||
| 8 | + | - | 1:45 | ||||||
| 14 | + | + | + | 1:360 | |||||
| pig No.050 |
5 | + | / | / | |||||
| 8 | + | - | 1:180 | ||||||
| 14 | + | + | + | 1:360 | |||||
Note: / undetected + positive - negative
dpi : days post infection NSP: nonstructural protein SP: structural protein
Ab: Antibody