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. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: Nat Commun. 2011 Nov 8;2:529. doi: 10.1038/ncomms1532

Fig. 3. miR-137 inhibits LSD1 expression.

Fig. 3

a. The nucleotide pairing of miR-137 and LSD1 3’ UTR. b. miR-137 represses luciferase reporter gene upstream of the LSD1 3′ UTR. Luciferase reporter gene under the control of wild type (WT) or mutant (MT) LSD1 3′ UTR was transfected along with control RNA (C) or miR-137 RNA duplexes into HEK 293 cells. s.d. is indicated by error bars. *p<0.005 by Student’s t-test. n=3. c. Western blot analysis of LSD1 and methylated histone H3K4 expression in control RNA (C) or miR-137-transfected neural stem cells. Histone H3 (H3) and GAPDH were included as controls. The molecular weight of each band is indicated at the left. d. Quantification of the Western blot results in panel c. The expression of each protein was normalized to the expression of GAPDH. miR stands for miR-137. *p<0.001 by Student’s t-test. n=3. e. Knockdown of LSD1 expression in miR-137-electroporated brains. E13.5 mouse brains were in utero electroporated with control RNA-RFP or miR-137-RFP. LSD1 expression was detected by immunostaining shown in green. Arrows indicate miR-137-electroporated cells exhibiting LSD1 knockdown. Scale bar, 10 µm.