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. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: Nat Commun. 2011 Nov 8;2:529. doi: 10.1038/ncomms1532

Fig. 4. Co-transfection of LSD1 without its 3’ UTR rescues miR-137-induced precocious outward migration.

Fig. 4

a. In utero electroporation of a LSD1 vector without its 3’ UTR along with miR-137 (miR-137+LSD 1) rescued miR-137-mediated decrease in cell proliferation in the ventricular zone (VZ) and subventricular zone (SVZ) of embryonic mouse brains. The electroporated cells were RFP+. Proliferating cells were labeled by Ki67. Scale bar, 50 µm. b. Electroporation of miR-137 and LSD1 led to rescue of miR-137-induced precocious outward cell migration. The transfected cells were shown red due to the expression of RFP marker. Merged images of RFP and doublecortin (DCX) staining were shown at the bottom. VZ: ventricular zone; CP: cortical plate. Scale bar, 100 µm. c. Percent of Ki67-positive cells out of RFP-positive cells (Ki67+RFP+/RFP+) in miR-137 and LSD1-electroporated brains (2) and miR-137 alone-electroporated brains (1). s.d. is indicated by error bars. * p<0.05 by Student′s t-test. n=3. d. Quantification of RFP-positive (RFP+) cells that were migrated to the CP. Error bars are s.d. of the mean. *p<0.05 by Student’s t-test. n=3. e. Enlarged images of immunostaining of miR-137-RFP or miR-137-LSD1-RFP-electroporated brain sections. CP: cortical plate. Scale bar, 10 µm.