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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Neuroimmunol. 2012 Jan 30;244(1-2):23–31. doi: 10.1016/j.jneuroim.2011.12.021

Fig. 1. Experimental design.

Fig. 1

On day 0 (A), baseline paw withdrawal latency (Radiant heat test) and paw withdrawal threshold (vonFrey filament test) values were measured for each rat. Subsequently, the animals received intraperitoneal (i.p.) injections of saline (200 μl) or morphine (5mg/kg in 200 μl) with/without AM 1241 (0.5–2.5mg/kg in 200 μl) and with/without a cannabinoid antagonist (AM 630; 600 μg/kg or AM 251 (600 μg/kg). The animals were tested for acute antinociception 30 min after drug administration (B). The injection regimen continued in each animal group for 6 days (C) twice daily (a total of 12 injections; Day 0 – Day 5). The animals were tested for paw withdrawal latencies and paw withdrawal thresholds every day in the morning (9:30) and 96 h (day 9) after the last drug treatment (D). The drug-withdrawn animals were subsequently euthanized and the spinal cords were isolated for immunohistochemistry.