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Neurite growth and synaptogenesis of OHCs are grossly unaffected in Prop1 df mutants. A Synaptophysin, a presynaptic marker of efferent fibers, is stained on whole mount preparations of cochlear epithelia with an anti-synaptophysin antibody (green). B Neurofilament protein (NF-200) immunostaining was used to detect the neurite outgrowth in cochlea whole mounts of P28 mutants as well as wild-type controls. Prestin immunostaining was used to indicate the position of OHCs (red).
