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Analysis of Cel01 production and purification by SDS PAGE (a) and Western blot analysis (b). His6-tagged Cel01 was purified from cell extract of E. coli BL21/pCel01 by nickel affinity chromatography. a Lanes: M, marker proteins; 1, crude extract of E. coli BL21/pCel01; 2, flow through fraction; 3, wash fraction; 4, eluate (further purified by ultrafiltration); b Lanes: M, marker proteins 5, crude extract; 6, purified Cel01
