Upregulation of SRSF5 affects alternative splicing of human hnRNP A/B. (A) Potential ESEs in exon 7 of human hnRNP A/B, based on the ESEfinder program. (B) Effect of overexpression of SRSF5, SRSF6 and SRSF2 on alternative splicing of human hnRNP A/B. Total RNA was extracted from untreated HeLa cells (lanes 1, 3, and 5) and from cells transfected with the indicated plasmids (lanes 2, 4, and 6) and analyzed by RT-PCR using the indicated primer pairs. The two isoforms of hnRNPA/B are schematically drawn on the left. The results represent three independent experiments.