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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Chem Biol. 2011 Nov;7(11):803–809. doi: 10.1038/nchembio.663

Figure 3.

Figure 3

Analysis of DRGs. (a) Immunostaining and fluorescent imaging of neurons and glia from dissociated E14 DRGs. Stimulated DRGs were imaged using a fluorescent TuJ1 antibody to label neurons (green), DAPI to label nuclei (blue), and fluorocoxib A to label COX-2 (red). (b) Western blot analysis of basal versus stimulated DRGs comparing enzymes involved in endocannabinoid metabolism and prostaglandin synthesis. (c) LC-MS chromatographic peaks and SRM transitions for prostaglandins derived from AA, AEA, and 2-AG isolated from DRGs.

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