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. Author manuscript; available in PMC: 2013 Jan 24.
Published in final edited form as: Biochemistry. 2012 Jan 17;51(3):820–828. doi: 10.1021/bi201731p

Figure 2. Inhibition of FVIII LC and FX binding by DEGR-APC.

Figure 2

FVIII LC (500 nM) was incubated with FX-coated wells (50 nM/well) in the presence of various concentrations of DEGR-APC. Bound FVIII LC was detected using a biotinylated anti-FVIII LC antibody, 10104. The absorbance value corresponding to FVIII LC bound to FX in the absence of DEGR-APC was defined 100%. The percentage of FVIII LC was plotted as a function of DEGR-APC concentration, and the data were fitted by nonlinear least squares regression using Equation 2 (competitive inhibition model). Experiments were performed at least three separate times and mean values are shown.