Table 1.
Details of 16 microsatellite loci developed for Chaetodon lunulatus by Lawton et al. (2010). Primer sequences and repeat motifs are provided as given by the authors of the original study (Lawton et al. 2010). Annealing temperatures (Ta) and size ranges are the ones utilized and found in the present investigation
| Locus (label) | Primer sequence (5′-3′) | Repeat motif | Ta (°C) | Size range (bp) |
|---|---|---|---|---|
| Lun01 (FAM) | TGAACTGCAAAGCAACAACC | (CAT)17 | 55 | 2 |
| CTGCTTCTCTTTGGTGAGGAG | ||||
| Lun03 (TET) | TGTGTGTCACCACCTGGTCT | (AG)29 | 58 | 175–239 |
| ACTCAGTTTTGAGCCGCTTC | ||||
| Lun05 (FAM) | GCAACCCAGTCTCACATCAA | (CAA)30 | 55 | 155–191 |
| TCTGCTATTTCACAATTTTAGAGCA | ||||
| Lun07 (HEX) | AAGTGCCCTTTAGCAAAGCA | (TG)17 | 58 | 153–212 |
| CTCCAGTCGCTTTCTGTGTG | ||||
| Lun08 (HEX) | GGCCTTTGTTTGTGGTCATT | (CA)26 | 55 | 174–226 |
| CCTGAAGAGAGAGCTGCTCAA | ||||
| Lun09 (TET) | CCTGTGTTTGTCATCCAACG | (TG)15 | 58 | 143–167 |
| CTTTGGGACACACACTTCCA | ||||
| Lun10 (TET) | TTGTGTTGTTTTAGTGTTCCCTTT | (AC)24 | 58 | 223–285 |
| TGAGTGGTTATGATACATTAGATTTTG | ||||
| Lun14 (HEX) | TACGTTGGACAGTGGCTGTG | (TCA)11 | 58 | 207–240 |
| TGGCTCTGTGGCATGTATGT | ||||
| Lun17 (*) | TCAGAGGTCGCTAACGTGTG | (GAT)12 | 55 | 1 |
| CTCTAACGCGTCCTCTGTCC | ||||
| Lun19 (FAM) | TCCAGTTCCATTCTGCCTTT | (GAT)16 | 55 | 125–188 |
| CCGTCATTAACCTCCAGCAG | ||||
| Lun20 (TET) | CAGTGTCGGAGAACAACGAA | (CTT)12 | 58 | 2 |
| TCACTGTGTCACCAATGCAC | ||||
| Lun21 (FAM) | CAGGGAAAATCACACTTTCACA | (TGCC)14 | 55 | 223–283 |
| TGTCAAGCTGTGTGGGACAT | ||||
| Lun22 (HEX) | GGATGATGCAACTGATGGAA | (ATC)15 | 58 | 2 |
| TGTAGCATTTCATCTTTGACACTG | ||||
| Lun29 (HEX) | CACCCACAGGCAGTGTATTG | (AC)33 | 55 | 233–277 |
| GCCAGCCTGTCAAAACTTTA | ||||
| Lun34 (TET) | CATGCTTGGGTGAGCATGTA | (CA)36 | 58 | 165–185 |
| TGTGCGTTTGTGCAAGTGTA | ||||
| Lun36 (FAM) | GCGTTTGACTTCACGTTTCA | (GT)30 | 58 | 188–238 |
| TGCAAAACAACAACCTACGG |
1
Discarded prior to genotyping.
2
Could not be scored reliably.