Table 3.
Effects of inhibitors of phosphodiesterase and nitric oxide synthase on responses to L-arg and SNP in carbachol (CARB)-precontracted monkey ciliary muscle (CM) strips.
| Agents, conc (M) | Maximum relaxation response % change in CARB ΔForce
|
n | Comparison | |||
|---|---|---|---|---|---|---|
| LONG
|
CIRC
|
|||||
| mean | sem | mean | sem | |||
| CARB/L-ARG 10−5 | −27.86* | 10.00 | −33.22* | 12.91 | 7 | vs CARB from L-arg concentration–response |
| CARB/IBMX (10−4) + L-ARG (10−5) | −22.91* | 2.93 | −24.39* | 3.74 | 7 | vs CARB + IBMX contraction |
| CARB/SNP 10−5 | −53.68* | 5.54 | −60.79* | 9.82 | 8 | vs CARB from SNP concentration–response data |
| CARB/SNP 10−4 | −76.95* | 8.22 | −83.53* | 10.75 | 9 | vs CARB from SNP concentration–response data |
| CARB/ODQ (10−4) + SNP (10−5) | −36.49* | 8.08 | −37.35* | 8.62 | 6 | vs CARB/ODQ contraction after ODQ pretreat |
| CARB/ODQ (10−4) + SNP (10−5) | −11.64 | 29.33 | −42.09* | 6.42 | 5 | vs CARB/ODQ contraction, simultaneous |
| CARB/ODQ (10−4) + SNP (10−5) | −25.19 | 13.77 | −39.51* | 5.32 | 11 | vs CARB/ODQ contraction; combined data |
| CARB/MB (10−5) + SNP (10−5) | −44.33* | 11.92 | −50.70* | 7.46 | 5 | vs CARB/MB contraction |
| CARB/MB (10−4) + SNP (5 × 10−5) | −50.03* | 9.50 | −72.47* | 8.85 | 4 | vs CARB/MB contraction |
L-arg and SNP responses without inhibitor are taken from the concentration–response curves in Fig. 2 for reference. Inhibitor data are expressed as the %Δ in the initial CARB contraction force (in the presence of the inhibitor) for each vector (longitudinal = LONG; circular = CIRC) versus the concentration of each compound. N is the number of different monkey CMs represented. A given CM may have received more than one concentration of a given agent. Bold values indicate values that are significant.
Significantly different from 0.0, paired t-test:
p < 0.05, minimum. Initial CARB contraction forces (mean ± S.E.M.) were as follows: L-arg, 66.6 ± 9.8 mg LONG, 40.2 ± 6.7 mg CIRC; L-arg + IBMX, 80.9 ± 5.1 mg LONG, 42.9 ± 5.6 mg CIRC; SNP, 80.0 ± 9.2 mg LONG, 43.8 ± 6.4 mg CIRC; SNP + ODQ (pretreat), 79.4 ± 12.2 mg LONG, 36.7 ± 6.6 mg CIRC; SNP + ODQ (simultaneous), 91.0 ± 33.0 mg LONG, 66.8 ± 29.3 mg CIRC; SNP + MB (10−5 M), 56.8 ± 13.2 mg LONG, 47.8 ± 9.9 mg CIRC; SNP + MB(10−4 M), 72.8 ± 13.6 mg LONG, 42.2 ± 9.0 mg CIRC.
ODQ pretreat, CM was incubated in ODQ alone prior to the addition of CARB + ODQ. ODQ + CARB simultaneous, ODQ and CARB were added to the CM simultaneously without ODQ preincubation.
Using the unpaired t-test: there was no difference between results with ODQ pretreat vs simultaneous (p > 0.39 for any combination of peak, trough, CIRC, LONG); there was borderline significance comparing combined ODQ data vs SNP 10−5 (0.05 < p < 0.1 for maximum relaxation in both LONG and CIRC vectors).