Fig. 6.

Ectopic expression of GATA6 or GATA4 is sufficient to restore the endoderm differentiation in Grb2 (−/−) ES cells in monolayer cultures. RW4 or Grb2 (−/−) ES cells were transfected with expression vectors for GATA4, GATA6, H2B-GFP, or vector control. Following transfection for 3 days, the cells were harvested for analysis by Western blots and immunofluorescence microscopy. A: Western blot analysis was performed for laminin, Dab2, GATA6, and GATA4 in the transfected ES cells. B: Immunostaining for GATA6 (green), GATA4 (red), and Dab2 (white) was performed simultaneously in the transfected cells. DAPI (blue) was used to mark the nuclei.