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. 2008 Nov 19;100(22):1592–1605. doi: 10.1093/jnci/djn379

Figure 3.

Figure 3

The association of overexpressed in lung cancer 1 (OLC1) overexpression with cigarette smoking and lung tumorigenesis. A) Representative examples of immunohistochemical (IHC) staining of OLC1 in lung squamous cell carcinoma (SCC) tissue samples from 35 lung SCC patients with various preneoplastic or neoplastic lesions using a rabbit polyclonal anti-OLC1 antibody. a, normal bronchial epithelia indicated by black arrows, negative; b, hyperplasia (black arrow, negative) and a primary SCC (open arrow, positive); c, severe dysplasia (open arrow, positive) and the nearby normal bronchial epithelia (black arrow, negative); d, carcinoma in situ (CIS, open arrow, positive) and the nearby normal bronchial epithelia (black arrows, negative); e, SCC nest, scattered cancer cells in the basal layer (open arrow, positive), and the adjacent normal epithelia (black arrow, negative); f, primary SCC of the lung, positive. Scale bars in a, c, e, and f represent 50 μm and those in b and d represent 100 μm. B) Results of IHC staining for OLC1, performed as described in (A), in lung SCC tissue samples from 35 lung SCC patients with various preneoplastic or neoplastic lesions. *Primary tumor vs normal bronchial epithelia: P < .001; primary tumor vs hyperplasia: P < .001; dysplasia/CIS vs hyperplasia: P < .001; dysplasia/CIS vs normal bronchial epithelia: P < .001. All P values were two-sided and calculated using generalized estimating equation (GEE) analysis, with 1 degree of freedom. C) OLC1 protein overexpression as detected by IHC in the paraffin-embedded tissue samples of primary lung SCCs from 371 patients and smoking history in these lung SCC patients. *P < .001 (two-sided), calculated using the Pearson chi-square test with 1 degree of freedom. D) Cigarette smoke condensate (CSC) treatment and OLC1 overexpression in MBE, H1299, and primary cultured normal human bronchial epithelial (PBE) cells. Immunoblot analysis of OLC1 expression using the OLC1 antibody in (A). A total of 60 μg of total protein was loaded from MBE and PBE cells, and 10 μg of total protein was loaded from H1299 cells. Blots were probed with mouse monoclonal anti–β-actin antibody as a control for protein loading and transfer. One representative blot from three independent experiments is shown.