Figure 1.

The gating strategy for enumeration of circulating PPCs. (a) Peripheral blood mononuclear cells were first gated into the CD45-negative fraction to exclude hematopoietic cells. (b) The total Syto16+ population of nucleated cells was selected to avoid inclusion of contaminating red cells, platelets, and debris in the analysis. (c, d) The resulting population was analyzed for expression of CD31 and CD140b. Panel (c) shows a case with low baseline PPCs (Syto16+CD45−CD31−CD140b+ cells), while (d) shows the same case 3 months after initiation of glucose control.