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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: J Neurosci Methods. 2011 Jan 12;196(1):99–106. doi: 10.1016/j.jneumeth.2011.01.004

Figure 5. Unchanged levels of phospho-proteins in cortical lysates from heat stabilized tissue and decreased levels in frozen tissue with room temperature incubation.

Figure 5

Signals from replicate Western blots as in Figure 4 were normalized to actin. Histograms represent average values for each group of samples relative to 100% for frozen tissues at 0 min RT (white bars); black bars, stabilized tissues 0′ RT; grey bars, frozen tissues 30′ RT; striped bars, stabilized tissues 30′ RT. Errors bars are the standard error of the mean. Statistical significance was determined by the unpaired Student's t-test. *p<0.05; **p <0.001:***p <0.0001.