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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: J Neurosci Methods. 2011 Jan 12;196(1):99–106. doi: 10.1016/j.jneumeth.2011.01.004

Table 1. Protocol comparisons, steps and time frames, post euthanasia to lysis.

ST, heat stabilized; FZ, frozen; FSH, fresh. RT, room temperature incubation. N/A, not applicable.

Protocol Remove
brain		 Dissect Heat Freeze Thaw Lyse,
0′ RT		 Lyse,
+30′ RT
ST 30″ N/A 50″, 95° Yes Yes, dissect ½ ½
FZ 30″ 90″, ice N/A Yes No ½ ½
FSH 30″ 90″, ice N/A N/A N/A ½ ½