A) Primers (ChIP-0) were designed to amplify by qPCR a sequence surrounding the NF-κB and AP-1 binding sites in the distal bovine MMP-9 promoter. The right arrow marks the transcription start site.
(B-F) BL3 and Theileria-infected TBL3 cells were incubated with/without Buparvaquone (BUP) for 64 hours. Chromatin Immunoprecipitation analysis with antibodies recognizing (B) H3K4me3, (C) H3K4me2, (D) Acetyl-H3, (E) H3K27me3 and (F) H3K9me3 marks. Error bars are representative of two independent experiments.
*p<0.05