A) HT1080 cells were transfected with two different siRNA oligonucleotides against SMYD3 or against MMP-9 or control siRNA (Ctrl), followed by analysis of cell migration on collagen-coated filters. Results are shown with respect to controls and error bars represent three independent experiments.
B) Western blot analysis showing the efficiency of siRNA MMP-9 knockdown in HT1080 cells. Beta-actin was used as control.
C) HEK293T cells were transfected with SMYD3-expressing vector or control vector (Ctrl). Exogenous SMYD3 protein levels were analysed by Western blot analysis and Beta-actin was used as control.
D) SMYD3 transfection into HEK293T cells caused an upregulation of endogenous MMP-9 levels as assessed by RT-qPCR analysis.
E) SMYD3 over-expression in HEK293T cells caused a significant increase in cell migration, compared to Control (Ctrl) transfected cells. Error bars represent three independent experiments.
*p<0.05, **p<0.01, ***p<0.001