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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cancer Res. 2011 Dec 22;72(3):810–820. doi: 10.1158/0008-5472.CAN-11-1052

Figure 6. SMYD3 modulation affects cell migration of human cell lines.

Figure 6

A) HT1080 cells were transfected with two different siRNA oligonucleotides against SMYD3 or against MMP-9 or control siRNA (Ctrl), followed by analysis of cell migration on collagen-coated filters. Results are shown with respect to controls and error bars represent three independent experiments.

B) Western blot analysis showing the efficiency of siRNA MMP-9 knockdown in HT1080 cells. Beta-actin was used as control.

C) HEK293T cells were transfected with SMYD3-expressing vector or control vector (Ctrl). Exogenous SMYD3 protein levels were analysed by Western blot analysis and Beta-actin was used as control.

D) SMYD3 transfection into HEK293T cells caused an upregulation of endogenous MMP-9 levels as assessed by RT-qPCR analysis.

E) SMYD3 over-expression in HEK293T cells caused a significant increase in cell migration, compared to Control (Ctrl) transfected cells. Error bars represent three independent experiments.

*p<0.05, **p<0.01, ***p<0.001