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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Genet. 2011 Oct 9;43(11):1147–1153. doi: 10.1038/ng.971

Fig.2. Disruption of the centrosomal gene CEP63 in vertebrate cells.

Fig.2

(A) WT and CEP63KO DT40 cells were co-stained with antibodies against CEP63 (red in merge) and γ-tubulin (green in merge). Magnified views of framed areas are shown in insets. DNA is blue. Scale bar=5μm. (B) Western blots of cytoplasmic cell extracts (CCE) from WT and gene-disrupted DT40 cell lines were probed with protein G antibodies. TAP cell lines contain in-frame GsTAP tag in their CEP63 alleles. α-tubulin serves as loading control. (C) Summary of the localisation and expression of tagged and truncated CEP63 products in the DT40 cell lines specified. n/a: the antibody epitope is largely destroyed in predicted protein product.