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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Genet. 2011 Oct 9;43(11):1147–1153. doi: 10.1038/ng.971

Fig.5. CEP63-dependent centrosomal accumulation of CEP152 maintains normal centrosome numbers.

Fig.5

(A) Localisation of STREP-tagged human CEP152 in transfected WT and CEP63KO DT40 cells. Magnified views of framed areas are shown in panels below. Cells are co-stained with antibodies against centrin-3 (green in merge) and CEP152 (red in merge). (B) Outline of constructs that were transfected into CEP63KO DT40 cells to derive cell lines stably expressing transgenes. (C) Cytoplasmic cell extracts of CEP63KO-derived DT40 cell lines (nomenclature as in B) were immunoblotted with antibodies against CEP152 and as a loading control, α-tubulin. Below mitotic cells co-stained with antibodies against centrin-3 (green in merge) and strep-tag II (red in merge) are shown. Magnified views of framed areas are shown. DNA is in blue. (D) Cytoplasmic cell extracts of CEP63KO-derived DT40 cell lines (nomenclature as in B) were immunoblotted with antibodies against CEP63 and as a loading control, α-tubulin. Below mitotic cells co-stained with antibodies against centrin-3 (green in merge) and CEP63 (red in merge) are shown. Magnified views of framed areas are shown. DNA is in blue. (E) Graph depicts quantification of spindle phenotypes in CEP63KO-derived DT40 cell lines (nomenclature as in B; n=2; >350 mitotic cells per clone). (F) Graph depicts number of centrioles in monastrol-induced monopoles of CEP63KO-c152 or CEP63KO-c152-pact DT40 cell lines (nomenclature as in B; n=2). Bars in graphs correspond to mean±s.d. p values were obtained by two-tailed, unpaired Student t test. Scale bars=5μm.