Figure 7.
Diprotin A enhances engraftment of donor cells in canine allogeneic transplantation. (A) Skeletal muscle cryosections from a chimeric cxmd canine (H376) biopsied 8, 16, and 24 weeks after injection with 5 × 105 donor cells in 1.2% BaCl2, or 5 × 105 donor cells treated with diprotin A in 1.2% BaCl2 were immunostained with anti-dystrophin and fluorescently labeled secondary antibodies. The number of fibers expressing canine dystrophin was determined and the points represent the average number of fibers expressing dystrophin ± SD (n = 5 cryosections from each biopsy). (B) Skeletal muscle cryosections from a chimeric cxmd canine (H220) biopsied 8, 16, and 24 weeks after injection with 4 × 105 donor cells or 4 × 105 donor cells treated with diprotin A were immunostained with anti-dystrophin and fluorescently labeled secondary antibodies. The number of fibers expressing canine dystrophin was determined and the points represent the average number of fibers expressing dystrophin ± SD (n = 5 cryosections from each biopsy).