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Thermostability of Thermobacillus xylanilyticus xylanase (Tx-Xyn) and mutants thereof
| Mutant | Tm (°C) | t1/2 at 60°C (h) |
|---|---|---|
| Tx-Xyn | 75.9 | 5.4 |
| Y6H | 72.9 | 2.6 |
| S27T | 76.4 | 6.4 |
| Y111H | 75.1 | 3.9 |
| Y6H-Y111H | 72.7 | 2.7 |
| S27T-Y111H | 75.4 | 6.4 |
| Y6H-S27T-Y111H | 74.3 | 3.9 |
| Y3W | 73.1 | 3.2 |
| Y111S | 75.1 | 3.6 |
| Y111T | 74.9 | 5.0 |
The melting temperature (Tm) was determined using differential scanning fluorimetry (DSF) and the half-life (t1/2) was defined as the period necessary for the initial activity to be reduced by 50% at 60°C.
