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. 2012 Mar 12;7(3):e32457. doi: 10.1371/journal.pone.0032457

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Lamarque et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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To follow RON10HA biosynthesis in time and space, co-localization experiments have been performed on Δron9-R10HA parasites or RON10HA parasites as a control. Anti-HA antibody was used to follow RON10 fate, while anti-RON9 (A) and anti-RON4 (B) were used to label the rhoptry neck, anti-IMC1 antibodies (C) and anti-ISP1 antibodies (D) allowed us to follow the endodyogeny process during cells replication. Anti-proROP4 antibodies allowed detection of pre-rhoptries (E) and co-transfection of DER1-GFP plasmid allowed detection of the ER (F). Scale bars = 5 µm.