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. 2012 Mar 12;7(3):e32457. doi: 10.1371/journal.pone.0032457

Figure 8. Δron9 parasites do not display any defect in replication and invasion in vitro, nor in virulence in vivo.

Figure 8

(A) The intracellular replication of Δron9 parasites was compared to that of the parental Δku80, 18 h post-infection. For this, the number of parasites per vacuole was counted after anti-SAG1 labeling of the parasite surface. Values represent means ± standard deviations (SD), n = 3, of 4 independent assays. (B) Invasion assays were carried out on freshly released highly synchronized tachyzoites. Invasion was allowed to take place for 5 min prior cells fixation and IFA processing as described in material and methods section. Values represent means ± standard deviations (SD), n = 3, from a representative experiment out of 3 independent assays. (C) Deletion of RON9 did not reduce virulence in mice. Twenty tachyzoites of the indicated strains were injected i.p. into Balbc mice (n = 20), and mouse survival was monitored daily for 15 d.