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. 2012 Mar 12;7(3):e32635. doi: 10.1371/journal.pone.0032635

Figure 6. Restoration of CRIM1 expression can rescue ß-catenin level in CRIM1 loss-of-function embryos.

Figure 6

(A–C) Whole-mount immunofluorescence labeling of stage 13 Xenopus embryos in which XLCAB MOs, MO-resistant mRNA of FLAG-tagged full-length CRIM1 (CRIM1-FL) or a combination of the two were injected at the 4-cell stage, together with dextran tracer. In (A and C) the asterisks indicate regions where labeling in the apical junctional complex were not imaged due to the optical sectioning plane. (D) Quantification of relative expression level of FLAG-tagged CRIM1 protein by calculating the ratio of average pixel intensity of FLAG labeling over 150 pixel line intervals placed in tracer-positive or tracer-negative regions (white bars in A–C). (E) Quantification of relative expression level of ß-catenin by calculating the ratio of average pixel intensity of ß-catenin labeling over 150 pixel line intervals placed in tracer-positive or tracer-negative regions (white bars in A–C).