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. 2012 Mar 12;7(3):e33007. doi: 10.1371/journal.pone.0033007

Figure 5. EGFP and GLuc expression in replicon-defective transfected cells infected with various viruses.

Figure 5

BHK-21 cells were transfected with pVaXJ-EGFPΔnsp4 or pVaXJ-GLucΔnsp4 using Lipofectamine 2000 reagent, 6 h before mock-infection or infection with 1 MOI of various viruses, including alphaviruses [SINV (XJ-160, YN87448, and MX10), CHIKV (SD08Pan), and GETAV (HB0234)], and non-alphaviruses [JEV (P3) and TAHV (XJ0625)]. (A–H) EGFP expression was examined 44 h post-infection. Green color indicates EGFP, and blue color indicates nucleus. (I) GLuc activity was measured 44 h post-infection. Each data point represents the mean ±SEM of three independent experiments. RLU, relative light units.