Figure 5. Detection of a Th1-dominant cellular response in GlgP-immunized mice.

Splenocytes harvested from EB (open bar) or GlgP (hatched bar) -immunized mice as described in Fig. 4 legend were in vitro re-stimulated with UV-inactivated Chlamydia muridarum EB organisms at 1×10⁶ IFUs per well, 10 µg/ml GlgP or medium alone (none) as indicated at the bottom of the figure. Three days after the stimulation, the culture supernatants were collected for IFNg and IL-5 detection and the results were expressed as pg or ng/ml as displayed along the Y-axis (mean ± SD). Note that both EB and GlgP-immunized mice produced much higher concentrations of IFNg than IL-5, indicating a Th1 dominant cellular response in theses mice.