Table 1. Real-time qPCR measurement of senescence-up-regulated genes (SURGs) and senescence-down-regulated genes (SDRGs) mRNA levels in tissue used for ChIP-seq.
| 23 d | 52 d | |
| SURGs | ||
| At5g45890, SAG12 | 1 | 90148 |
| At1g29640 | 1 | 635 |
| At3g44300 | 1 | 309 |
| At5g13080 | 1 | 304 |
| At1g73220 | 1 | 113 |
| At2g29460 | 1 | 99 |
| At5g42800 | 1 | 74 |
| At1g13340 | 1 | 16 |
| SDRGs | ||
| At2g10940 | 14116 | 1 |
| At3g16670 | 2788 | 1 |
| At5g26000 | 70 | 1 |
| At3g05730 | 55 | 1 |
| At5g25460 | 53 | 1 |
| At3g27690, Lhcb2.3 | 25 | 1 |
| At3g44990 | 0.4 | 1 |
Total RNA was harvested from the same leaves used in the ChIP-seq analysis, and real-time qPCR was performed using ACT2 as a reference. SURGs and SDRGs were identified from published microarray data [4]. Fold-induction at 52 d for SURGs and at 23 d for SDRGs is reported. Eight of 8 tested SURGs were up-regulated by at least 15-fold, while 6 of 7 tested SDRGs were down-regulated by at least 24-fold.